6-Azidohexanoic acid is primarily used in chemical synthesis and bioconjugation applications. It serves as a versatile linker or spacer molecule in the development of bioactive compounds, particularly in the field of bioconjugation, where it facilitates the attachment of biomolecules like proteins, peptides, or antibodies to other functional groups or surfaces. The azido group in its structure allows for efficient click chemistry reactions, such as the copper-catalyzed azide-alkyne cycloaddition (CuAAC), enabling precise and stable connections between molecules. This makes it valuable in drug delivery systems, diagnostic assays, and materials science for creating functionalized surfaces or polymers. Additionally, it is utilized in research for labeling and tracking biomolecules in cellular and molecular studies.