Lys05 is primarily used in research settings to study autophagy, a cellular process that involves the degradation and recycling of cellular components. It acts as a potent autophagy inhibitor by targeting lysosomal activity, making it a valuable tool for understanding the role of autophagy in various diseases, including cancer, neurodegenerative disorders, and infectious diseases. Researchers utilize Lys05 to investigate how blocking autophagy affects tumor growth, as it has shown potential in enhancing the efficacy of chemotherapy by preventing cancer cells from surviving under stress conditions. Additionally, it is employed in studies exploring the relationship between autophagy and cellular metabolism, as well as in drug development efforts aimed at modulating autophagy for therapeutic purposes.