Lys05 is primarily utilized in biomedical research, particularly in the study of autophagy, a cellular process involved in the degradation and recycling of damaged or unnecessary cellular components. It acts as a potent inhibitor of autophagy by targeting lysosomal activity, making it a valuable tool for understanding the role of autophagy in various diseases, including cancer, neurodegenerative disorders, and infectious diseases. Researchers use Lys05 to investigate how autophagy modulation can influence disease progression, treatment resistance, and cellular survival. Its application extends to preclinical studies, where it helps identify potential therapeutic strategies by blocking autophagy pathways in experimental models.