ROC-325 is primarily used in the field of oncology research, where it plays a significant role in studying autophagy, a cellular degradation process. It is particularly effective in inhibiting autophagy by targeting specific pathways, making it a valuable tool for understanding the mechanisms of cancer cell survival and resistance to treatments. Researchers utilize ROC-325 to explore how blocking autophagy can enhance the efficacy of chemotherapy and radiation therapy in various cancer models. Additionally, it aids in investigating the potential of autophagy inhibition as a therapeutic strategy for cancers that are heavily reliant on this process for growth and survival. Its application extends to preclinical studies, where it helps in identifying new drug targets and developing combination therapies to improve cancer treatment outcomes.