Tentoxin is primarily used in agricultural research to study its effects on plant physiology, particularly in understanding chloroplast function and photosynthesis. It is employed as a selective herbicide in experimental settings to target and inhibit the growth of certain plant species, making it a valuable tool for investigating plant-pathogen interactions and developing disease-resistant crops. Additionally, tentoxin serves as a model compound in biochemical studies to explore ATP synthase inhibition and its impact on energy metabolism in plants. Its ability to induce chlorosis in susceptible plants makes it useful for studying leaf discoloration and cellular stress responses. Researchers also utilize tentoxin to explore its potential in controlling weed populations and managing plant diseases in a controlled manner.