Used for detecting DNA fragmentation in apoptotic cells, this assay is widely applied in cancer research, developmental biology, and toxicology studies. The red fluorescence at 594 nm allows clear visualization of apoptotic nuclei under a fluorescence microscope, enabling easy distinction from other markers in multicolor staining. It is commonly used to assess cell death in tissue sections, cultured cells, and paraffin-embedded samples. The kit’s high sensitivity and specificity make it ideal for evaluating the effectiveness of chemotherapeutic agents, studying neurodegenerative diseases, and analyzing embryonic development. Its compatibility with flow cytometry also allows quantitative analysis of apoptosis in cell populations.