This compound, GS-441524, is the active nucleoside analog and key metabolite of the antiviral drug remdesivir. It exhibits potent antiviral activity against RNA viruses, including coronaviruses such as SARS-CoV-2 (causing COVID-19), Ebola virus, and hepatitis C virus. By incorporating into viral RNA and inhibiting RNA-dependent RNA polymerase, it effectively suppresses viral replication, reduces viral load, and improves clinical outcomes in preclinical and animal models. As an important intermediate in remdesivir synthesis, which received emergency use authorization for COVID-19 treatment, GS-441524 is a valuable tool in antiviral research and drug development. Ongoing studies assess its safety, efficacy, and potential for direct therapeutic applications against emerging viral threats.