HIV-1 Nef-IN-1 is primarily used in research focused on understanding and combating HIV-1 infection. It specifically targets the Nef protein, which plays a critical role in the virus's ability to evade the immune system and enhance its pathogenicity. By inhibiting Nef, this compound helps researchers study the mechanisms by which HIV-1 disrupts cellular functions and immune responses. It is a valuable tool in the development of potential therapeutic strategies aimed at blocking Nef activity, thereby reducing viral replication and improving immune system function in infected individuals. Its application is largely confined to laboratory settings, where it aids in the exploration of novel antiviral treatments and the broader understanding of HIV-1 biology.