NITD-2 is primarily used in research settings as an antiviral agent, particularly in studies focused on combating flaviviruses. It has shown potential in inhibiting the replication of viruses such as dengue, Zika, and West Nile virus. Researchers utilize NITD-2 to explore its mechanism of action, which involves targeting the viral RNA-dependent RNA polymerase, a critical enzyme for viral replication. This compound is valuable in the development of antiviral therapies and contributes to understanding viral pathogenesis. Its application is confined to laboratory studies and preclinical investigations, with the aim of advancing treatments for flavivirus infections.