Floxacrine is primarily used as a topical antiseptic and antimicrobial agent, especially in the treatment of skin and mucous membrane infections. It is effective against a broad spectrum of bacteria, including both Gram-positive and Gram-negative strains, making it suitable for use in wound care and burn treatments. Due to its ability to intercalate into microbial DNA and inhibit replication, it also shows activity against certain fungi and viruses. Floxacrine is commonly formulated in creams, ointments, and solutions for localized application, particularly in managing infected wounds and preventing secondary infections in dermatological conditions. Its low systemic absorption enhances its safety profile for long-term topical use.