Mito-TEMPO is widely used in research to target and mitigate oxidative stress within mitochondria, which are the energy-producing organelles in cells. Its application is particularly significant in studies focused on neurodegenerative diseases, cardiovascular conditions, and aging, where mitochondrial dysfunction and excessive reactive oxygen species (ROS) play a critical role. By selectively accumulating in mitochondria, it helps neutralize ROS, thereby protecting cells from oxidative damage. This makes it a valuable tool in understanding and potentially treating diseases linked to mitochondrial oxidative stress. Additionally, it is utilized in experimental models to explore its protective effects against ischemia-reperfusion injury, a common complication in organ transplantation and stroke.