TRX-RED is widely used in redox signaling research due to its ability to modulate cellular thiol-disulfide balance. It plays a critical role in studying oxidative stress responses, as it supports the function of antioxidant enzymes and helps maintain protein thiols in their reduced state. This makes it valuable in investigating conditions like inflammation, aging, and neurodegenerative diseases. It is also employed in cell culture systems to stabilize redox-sensitive proteins and transcription factors, enhancing experimental accuracy in molecular biology and drug development studies. Additionally, TRX-RED has been utilized in assays detecting reactive oxygen species, contributing to the evaluation of new therapeutic compounds with antioxidant properties.