Pefloxacin mesylate is widely used as an antibacterial agent, particularly in the treatment of infections caused by susceptible bacteria. It is effective against a range of Gram-negative and Gram-positive organisms, making it suitable for treating urinary tract infections, respiratory tract infections, skin and soft tissue infections, and bone and joint infections. It is also employed in managing gastrointestinal infections and septicemia. Due to its good tissue penetration, it is often used in cases where the infection is deep-seated or difficult to treat with other antibiotics. Additionally, it is sometimes used prophylactically in surgical procedures to prevent postoperative infections. Its broad-spectrum activity and bioavailability make it a valuable option in clinical settings.