Pseudolaric acid D is primarily studied for its potential antifungal and anticancer properties. It has shown inhibitory effects against various fungal strains, making it a candidate for development in antifungal therapies. In cancer research, it demonstrates activity by interfering with cell cycle progression and inducing apoptosis in certain tumor cell lines. Its ability to disrupt microtubule formation contributes to its cytotoxic effects, particularly in drug-resistant cancer cells. Due to these properties, Pseudolaric acid D is investigated as a lead compound for new chemotherapeutic agents. It also serves as a chemical probe in studying cell division mechanisms and microtubule dynamics in biological systems.