Demethoxydeacetoxypseudolaric acid B is a natural diterpenoid derivative isolated from the root bark of Pseudolarix kaempferi. It is primarily used in biomedical research for its multifaceted bioactivities, including potent antifungal and antibacterial properties against various pathogens, particularly skin-related fungi and bacteria. Studies have demonstrated its potential as a topical antimicrobial agent by inhibiting the growth of dermatophytes and other skin pathogens. Additionally, it exhibits anti-inflammatory effects in models of skin irritation, dermatitis, and other inflammatory conditions. The compound has also been investigated for anti-cancer properties, showing cytotoxicity against tumor cell lines through mechanisms such as microtubule disruption and inhibition of cell proliferation, making it a candidate for anti-cancer drug development. Due to its natural origin and bioactivity, it is explored in cosmetic formulations for antimicrobial and soothing effects. Currently limited to laboratory and preclinical studies; not approved for clinical use.