Pityrogrammin is primarily used in research settings for its biological activity, particularly in studies related to cell signaling and enzyme inhibition. It has shown potential as an antimicrobial agent, with activity against certain Gram-positive bacteria, making it a subject of interest in the development of new antibiotics. Additionally, it exhibits antifungal properties, which are being explored for agricultural applications to control plant pathogens. Due to its unique structure, it also serves as a natural product scaffold in synthetic chemistry for designing derivatives with enhanced bioactivity. Its role in ecological interactions, such as plant defense mechanisms, is another area of ongoing study.