Phenazine-1-carboxylic acid is widely used as a biopesticide in agriculture due to its strong antifungal and antibacterial properties. It effectively controls soil-borne plant pathogens such as Rhizoctonia solani, Fusarium species, and other fungi that cause root rot and damping-off diseases. The compound works by disrupting cellular redox processes in target pathogens, leading to reactive oxygen species accumulation and cell death.

It is particularly valuable in sustainable farming as it is derived from natural bacterial sources, such as Pseudomonas species, making it an environmentally friendly alternative to synthetic pesticides. Formulations containing this compound are applied as seed treatments, soil drenches, or foliar sprays to protect crops like rice, wheat, and vegetables.

In addition to agricultural uses, Phenazine-1-carboxylic acid shows promise in pharmaceutical research for its antimicrobial and potential anticancer activities, though these applications are still under investigation.