Desglucohellebrin is primarily used in scientific research, particularly in the study of cardiac glycosides and their biological activity. It serves as an intermediate in the synthesis of related compounds with potential pharmacological effects on heart function. Due to its structural similarity to other cardenolides, it is investigated for its influence on ion transport across cell membranes, especially sodium-potassium ATPase inhibition. This property makes it useful in understanding the mechanisms of heart muscle contraction and arrhythmias. Additionally, it has been explored for its cytotoxic effects in certain cancer cell lines, supporting its role in oncology-related research. Its applications remain mostly confined to laboratory and experimental settings, with limited use in clinical or industrial contexts.