Apramycin sulfate salt is widely used in veterinary medicine as an antibiotic to treat bacterial infections in livestock, particularly in pigs, cattle, and poultry. It is effective against Gram-negative bacteria, with some activity against Gram-positive, and is often administered via injection, feed additives, or water medication to combat gastrointestinal, respiratory infections, and those caused by antibiotic-resistant bacteria. In aquaculture, it is utilized to control bacterial diseases in fish and shrimp, promoting healthier stock and improving yields. Additionally, it is employed in research settings as a selective agent in molecular biology experiments, particularly in the study of bacterial resistance mechanisms. Its broad-spectrum activity, low toxicity, and role in preventing disease spread in farms make it a valuable tool in animal health, aquaculture, and scientific studies.