Kanamycin disulfate salt is widely used in microbiological research as an antibiotic to inhibit the growth of bacteria. It is particularly effective against Gram-negative and some Gram-positive bacteria, making it a valuable tool in laboratory settings for selecting and maintaining bacterial strains with specific resistance markers. In molecular biology, it is often used in the preparation of culture media to ensure the growth of only those bacteria that have been genetically modified to resist its effects. Additionally, it plays a role in the study of bacterial resistance mechanisms and the development of new antibiotics. In clinical applications, it is utilized in certain diagnostic tests to identify bacterial infections and determine appropriate treatment strategies. Its stability and effectiveness in various formulations also make it suitable for use in veterinary medicine to treat bacterial infections in animals.