Globomycin is primarily used in research settings to study bacterial cell wall synthesis and lipoprotein processing. It specifically inhibits the signal peptidase II enzyme, which is crucial for the maturation of lipoproteins in bacteria. This makes it a valuable tool for understanding bacterial physiology and developing potential antibiotics. Researchers utilize globomycin to investigate Gram-negative bacteria, as it disrupts their outer membrane integrity, providing insights into novel drug targets. Additionally, it aids in exploring resistance mechanisms and the role of lipoproteins in bacterial pathogenesis. Its application extends to screening for compounds that can enhance its antibacterial activity, contributing to the development of combination therapies against resistant bacterial strains.