Danofloxacin mesylate is primarily used in veterinary medicine as an antibacterial agent. It is effective against a wide range of bacterial infections in animals, particularly in livestock such as cattle, pigs, and poultry. The compound is commonly administered to treat respiratory infections, including bovine respiratory disease caused by Mannheimia haemolytica, Pasteurella multocida, and Haemophilus somnus. It is also used to combat colibacillosis in poultry, a condition caused by Escherichia coli.

The drug is typically administered via injection or orally, ensuring rapid absorption and distribution throughout the body. Its broad-spectrum activity makes it a valuable tool in controlling bacterial outbreaks in animal populations, thereby improving animal health and productivity. Additionally, danofloxacin mesylate helps reduce the risk of zoonotic diseases, which can be transmitted from animals to humans, contributing to overall public health safety. Proper dosage and adherence to withdrawal periods are essential to minimize the risk of antibiotic resistance and ensure food safety.