Nonactin is widely used as an ionophore, particularly for transporting potassium ions across biological membranes. Its ability to selectively bind and transport potassium ions makes it valuable in research studies involving membrane transport mechanisms and ion selectivity. It is also employed in biochemical assays to study cellular processes that depend on potassium ion gradients, such as nerve impulse transmission and muscle contraction. Additionally, Nonactin has been utilized in the development of ion-selective electrodes for detecting potassium ions in various samples, including biological fluids and environmental samples. Its role in modulating ion permeability has also made it a tool in investigating mitochondrial function and cellular energy metabolism.