N-Acetyl Sulfamethazine is the primary metabolite of Sulfamethazine, a sulfonamide antibiotic used in veterinary medicine. It is not used directly as an antimicrobial agent but serves as a key compound in pharmacological and biochemical research to understand the metabolism of sulfa drugs in animals. As an analytical reference standard, it is employed in laboratory analyses to detect and quantify Sulfamethazine and its metabolites in biological samples, such as urine, blood, or tissues from livestock. This application is essential for residue monitoring to ensure food safety, regulatory compliance, and withdrawal periods in animal husbandry. Additionally, it supports drug development studies by evaluating pharmacokinetics, excretion patterns, and safety profiles of sulfonamides in animal models.