Scillascillin is primarily used in research settings for its antibiotic properties, particularly in studies involving bacterial cell wall synthesis. It demonstrates activity against certain gram-positive bacteria by inhibiting peptidoglycan cross-linking, making it a useful tool in microbiology and antibiotic development research. Due to its structural similarity to other beta-lactam antibiotics, it is sometimes employed in comparative studies to understand resistance mechanisms, including beta-lactamase enzyme activity. Its application remains mostly confined to laboratory use rather than clinical treatment.