Used primarily as a selective agent in cell culture to eliminate non-transfected cells when working with puromycin resistance genes. Commonly applied in stable cell line generation, allowing researchers to maintain selective pressure to ensure only genetically modified cells survive. Also utilized in studies involving protein synthesis inhibition, as it interferes with ribosomal function, making it valuable in research on translation mechanisms and apoptosis. Frequently used in virology and cancer research for its ability to enrich transduced or transfected populations in both adherent and suspension cell types.