Amphotericin B methyl ester is a lipophilic derivative of the antifungal agent Amphotericin B, primarily used in research settings. It targets fungal cell membranes by binding to ergosterol, disrupting membrane integrity and leading to fungal cell death. Valued for its enhanced solubility in organic solvents and improved membrane penetration compared to the parent compound, it is commonly employed to study sterol interactions, membrane biology, and antifungal mechanisms. As an intermediate, it serves in the synthesis of novel lipid-based antifungal formulations with potentially reduced toxicity. Not approved for routine clinical use, its applications are limited to experimental and laboratory contexts, often via intravenous or liposomal delivery in preclinical studies.