Isopedicin is primarily used in the pharmaceutical industry for its antimicrobial properties. It shows activity against certain Gram-positive bacteria and has been investigated for potential use in treating bacterial infections resistant to conventional antibiotics. Research also explores its role as a lead compound in developing new semi-synthetic derivatives with enhanced stability and efficacy. Due to its specific mode of action, it is studied in combination therapies to reduce antibiotic resistance development. Additionally, Isopedicin finds limited use in laboratory settings as a selective agent in microbial culture media for isolating resistant strains.