Kirromycin is primarily used in biochemical research as a potent antibiotic that inhibits protein synthesis. It specifically targets the elongation factor Tu (EF-Tu) in bacteria, making it a valuable tool for studying the mechanism of protein biosynthesis. Researchers utilize kirromycin to explore the interactions between EF-Tu and ribosomes, as well as to investigate the translation process in prokaryotic systems. Additionally, its ability to bind EF-Tu with high affinity allows for the development of assays to screen for novel antibiotics or to study resistance mechanisms. In microbiology, kirromycin is employed to selectively inhibit bacterial growth in experimental setups, aiding in the understanding of bacterial physiology and the development of antimicrobial strategies. Its unique mode of action also makes it a candidate for studying the structural and functional aspects of EF-Tu in various bacterial species.