NH125 is primarily used in research settings as a potent and selective inhibitor of bacterial protein synthesis. It targets the initiation phase of translation by inhibiting the activity of the initiation factor IF2, making it a valuable tool for studying bacterial gene expression and ribosome function. Due to its specificity, NH125 is employed in antimicrobial development studies, particularly in exploring novel pathways to combat resistant bacterial strains. It has also shown activity against certain persistent forms of bacteria, including dormant or slow-growing populations that are typically less responsive to conventional antibiotics. This property makes NH125 useful in investigations aimed at developing treatments for chronic or recurrent infections. Additionally, it serves as a molecular probe in cellular assays to dissect signaling pathways related to stress response and survival mechanisms in microbial cells.