Hardwickiic acid is used in traditional medicine for its anti-inflammatory and antimicrobial properties. It has shown potential in inhibiting the growth of certain pathogenic bacteria and fungi, making it valuable in developing natural preservatives and topical antiseptics. Research also indicates its use in managing metabolic disorders due to its ability to influence lipid metabolism. Additionally, it serves as a phytochemical marker in the standardization of herbal extracts, particularly in plants used for liver protection and digestive health. Its role in plant defense mechanisms also makes it of interest in agricultural research for developing natural pest-resistant crops.