β-Anhydrouzarigenin is a bufadienolide aglycone used in organic synthesis as an intermediate for preparing cardiac glycoside derivatives with cardiotonic activity. It holds promise in pharmaceutical research due to its structural similarity to natural bufadienolides from plants like Strophanthus uzarensis, particularly in studies on positive inotropic, antiarrhythmic, and vasodilatory effects. It is investigated for developing therapeutic agents targeting cardiovascular disorders, such as heart failure and arrhythmias. Additionally, it is employed in structure-activity relationship (SAR) studies to improve the efficacy and safety of novel heart medications.