2,4,5-Trimethoxyaniline is primarily used as an intermediate in the synthesis of various organic compounds, notably in the production of trimethoprim, an antimicrobial agent combined with sulfamethoxazole for treating bacterial infections. It plays a role in pharmaceutical manufacturing and is also utilized in the creation of dyes and pigments, contributing to colors in textiles and industrial materials. Its application extends to research laboratories as a building block for synthesizing complex molecules in organic chemistry studies.