Testosterone acetate is primarily used in hormone replacement therapy (HRT) for individuals with low testosterone levels, helping to restore normal levels and alleviate symptoms such as fatigue, low libido, and muscle loss. It is also employed in the treatment of certain medical conditions like delayed puberty in males and hypogonadism. In veterinary medicine, it is utilized to enhance growth and improve muscle mass in livestock. Additionally, it has applications in research settings for studying androgen receptor function and hormonal regulation. Use should be under medical supervision due to potential side effects.