Neomycin sulfate is widely used as an antibiotic in both human and veterinary medicine. It is primarily applied topically to treat superficial skin infections, burns, and wounds, effectively combating bacterial growth. In ophthalmology, it is used in eye drops to treat conjunctivitis and other eye infections. Additionally, it is often included in combination creams with other antibiotics or corticosteroids to enhance its therapeutic effects. In veterinary practice, it is used to treat infections in animals, particularly in livestock. Neomycin sulfate is also employed in the preparation of certain pharmaceutical products, such as ear drops, to address ear infections. Its broad-spectrum antibacterial properties make it a valuable tool in preventing and managing infections across various applications.