AOZ is widely used as a marker residue in the detection of nitrofuran antibiotics in food products, particularly in meat, seafood, and poultry. It helps ensure food safety by monitoring compliance with regulatory standards, as nitrofurans are banned in many countries due to their potential carcinogenic effects. In analytical chemistry, AOZ is employed in methods like ELISA and LC-MS/MS for sensitive and accurate detection. Its application is critical in preventing the illegal use of nitrofurans in animal husbandry and safeguarding public health.