Acetonitrile is widely used as a solvent in the purification of butadiene in refineries due to its ability to selectively extract butadiene from hydrocarbon streams. It is also a key solvent in the pharmaceutical industry, particularly in the synthesis of antibiotics and vitamins, where it facilitates reactions and purification processes. In the field of analytical chemistry, acetonitrile is a preferred solvent for high-performance liquid chromatography (HPLC) because of its low viscosity and UV transparency, which enhance separation efficiency and detection sensitivity. Additionally, it serves as a solvent in the manufacturing of batteries, especially lithium-ion types, where it is used in the preparation of electrolytes. Acetonitrile is also employed in DNA synthesis and peptide sequencing, aiding in the precise assembly of nucleotides and amino acids. Its applications extend to the production of synthetic fibers and plastics, where it acts as a reaction medium or solvent.