Hexadecyltrimethylammonium hydroxide is widely used in various industrial and research applications due to its surfactant properties. It is commonly employed in the formulation of cleaning products, where it acts as a cationic surfactant to effectively remove dirt, grease, and oils from surfaces.

In the field of material science, it is utilized as a templating agent in the synthesis of mesoporous materials, helping to create structured materials with controlled pore sizes. Additionally, it finds application in the preparation of nanoparticles, where it aids in stabilizing and controlling the size and shape of the particles.

In biological research, it is used as a disinfectant and antimicrobial agent, particularly in laboratory settings to ensure sterile conditions. Its ability to disrupt microbial cell membranes makes it effective against a broad range of microorganisms.

Furthermore, it is employed in the textile industry as a softening agent for fabrics, improving their feel and texture. Its cationic nature allows it to bind effectively to negatively charged fibers, providing long-lasting softness.

In paper production, it helps improve quality and strength. In the petroleum industry, it serves as an aid in drilling to enhance the efficiency of drilling fluids.

Overall, its versatility as a surfactant and its ability to interact with various materials make it a valuable chemical in multiple industries.