Used primarily in research to study vitamin B6 deficiency, as it acts as an antagonist to pyridoxine, inhibiting its biological functions. It is employed in experimental models to understand the metabolic and neurological effects of B6 deficiency, particularly in cell culture and animal studies. Additionally, it has been utilized in cancer research to investigate the role of vitamin B6 in tumor growth and cellular metabolism. Its application extends to exploring the impact on neurotransmitter synthesis and immune system modulation.