Arecoline hydrobromide is primarily used in research settings as a cholinergic agent to study the effects of acetylcholine receptor activation. It is often employed in neurological and pharmacological studies to investigate the role of the parasympathetic nervous system, as it mimics the action of acetylcholine. Additionally, it has been utilized in experimental models to explore its impact on cognitive functions, memory, and behavior, including potential applications in models of Alzheimer's and Parkinson's diseases. In veterinary medicine, it has been applied as an anthelmintic to treat parasitic infections in animals. However, its use is limited due to its potential toxicity and side effects.