Used extensively as a chromogenic substrate in enzyme-linked immunosorbent assays (ELISA), it helps detect the presence of antibodies or antigens by producing a blue color upon oxidation. This color change is easily measurable, making it a valuable tool in medical diagnostics and research. Additionally, it is employed in forensic science for detecting blood traces due to its sensitivity to peroxidase activity. In industrial settings, it serves as a reagent in quality control processes to monitor the presence of specific enzymes or contaminants. Its stability and reliable performance make it a preferred choice in various analytical applications.