3,3',5,5'-Tetramethylbenzidine
≥99%(HPLC)
Reagent
Code: #71573
Alias
3,3′,5,5′-Tetramethylbenzidine
CAS Number
54827-17-7
science Other reagents with same CAS 54827-17-7
blur_circular Chemical Specifications
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Molecular Information
Weight
240.34 g/mol
Formula
C₁₆H₂₀N₂
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Registry Numbers
EC Number
259-364-6
MDL Number
MFCD00007748
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Physical Properties
Melting Point
168-171 °C(lit.)
Boiling Point
100 °C
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Storage & Handling
Storage
2~8°C
description Product Description
Widely used as a chromogenic substrate in enzyme-linked immunosorbent assays (ELISAs) for detecting peroxidase activity. It produces a blue color upon oxidation, which can be measured spectrophotometrically to quantify target analytes. Commonly applied in clinical diagnostics for detecting antibodies, antigens, or hormones in biological samples. Also utilized in immunohistochemistry to visualize peroxidase-labeled antibodies in tissue sections. Its sensitivity and stability make it a preferred choice in various biochemical and medical research applications.
format_list_bulleted Product Specification
| Test Parameter | Specification |
|---|---|
| Loss on Drying | <=0.5% |
| Melting point | 167-172 |
| Purity (HPLC) | 99-100% |
| APPEARANCE | White to yellow solid |
| Infrared Spectrum | Conforms to Structure |
| Peroxidase Detection | PASS |
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