Used primarily in gel filtration chromatography to separate molecules based on size. Commonly employed in protein purification processes to remove salts, small molecules, or other impurities from larger biomolecules like proteins or nucleic acids. It is also utilized in desalting and buffer exchange applications, making it a valuable tool in biochemistry and molecular biology labs. Additionally, it finds use in the preparation of samples for downstream analytical techniques such as electrophoresis or mass spectrometry. Its porous structure allows efficient separation while maintaining the integrity of sensitive biomolecules.