Oxolinic acid is primarily used as an antibacterial agent in veterinary medicine, particularly for treating bacterial infections in fish and poultry. In aquaculture, it is effective against gram-negative bacteria, helping to control diseases such as furunculosis in salmonids and other bacterial infections in fish farms. It is often administered through medicated feed to ensure proper dosage and efficacy. Additionally, oxolinic acid is utilized in the treatment of urinary tract infections in animals, providing a targeted approach to combat bacterial growth. Its application is carefully regulated to minimize the risk of antibiotic resistance and ensure safe use in animal husbandry.