Sephadex® G50 is widely used in biochemistry and molecular biology for the separation and purification of biomolecules based on their size. It is particularly effective for desalting, buffer exchange, and fractionation of proteins, peptides, and nucleic acids. In gel filtration chromatography, it separates larger molecules from smaller ones, making it a valuable tool for purifying samples before further analysis. It is also used in the removal of small molecules like salts, dyes, or unincorporated nucleotides from reaction mixtures. Additionally, Sephadex® G50 is employed in the preparation of samples for techniques such as electrophoresis, mass spectrometry, or sequencing, ensuring cleaner and more accurate results. Its versatility and reliability make it a staple in laboratory workflows for molecular separation and purification.