Neomycin sulfate is widely used as an antibiotic in both human and veterinary medicine. It is particularly effective against a range of Gram-negative and some Gram-positive bacteria. In humans, it is commonly applied topically to treat skin infections, burns, and wounds, often in the form of creams, ointments, or eye drops. It is also used in combination with other antibiotics to prevent infections in surgical settings. In veterinary medicine, neomycin sulfate is used to treat bacterial infections in animals, including gastrointestinal infections in livestock. It is often administered orally or as an injection. Additionally, it is used in animal feed to promote growth and prevent bacterial diseases. Neomycin sulfate is also employed in research laboratories as a selective agent in cell culture to prevent bacterial contamination. Its broad-spectrum antibacterial properties make it a valuable tool in maintaining sterile conditions in experimental setups.