Used as a reference standard in analytical chemistry for the calibration of instruments such as gas chromatographs (GC) and high-performance liquid chromatography (HPLC) systems. It ensures accurate identification and quantification of 1,2,4-trimethylbenzene in environmental samples, such as air, water, and soil, particularly in pollution monitoring and industrial hygiene testing. Commonly employed in volatile organic compound (VOC) analysis to assess emissions from petrochemical processes, fuel combustion, and solvent use. Its precise concentration allows for reliable quality control in laboratories performing regulatory compliance testing.