Used as a reference standard in analytical chemistry for the calibration of instruments such as gas chromatographs (GC) and high-performance liquid chromatography (HPLC) systems. It ensures accurate identification and quantification of 1,2,4-trimethylbenzene in environmental samples, air quality monitoring, and industrial hygiene testing. Commonly applied in detecting volatile organic compounds (VOCs) in soil, water, and ambient air, especially in regulatory compliance and pollution studies. Also utilized in research and quality control laboratories for method validation and performance verification of analytical procedures.