Used as a reference standard in analytical laboratories for the calibration of instruments such as gas chromatographs (GC) and high-performance liquid chromatographs (HPLC). Ensures accurate identification and quantification of 1,2,4-trimethylbenzene in environmental samples, air quality monitoring, and industrial hygiene testing. Commonly applied in the analysis of volatile organic compounds (VOCs) in soil, water, and air to assess contamination levels and ensure compliance with environmental regulations. Also utilized in research and development for method validation and quality control in chemical and petrochemical industries.