Used as a reference standard in analytical chemistry for the calibration of instruments such as gas chromatographs (GC) and high-performance liquid chromatography (HPLC) systems. It ensures accurate identification and quantification of 1,2,4-trimethylbenzene in environmental samples, air quality monitoring, and industrial hygiene testing. Commonly applied in laboratories analyzing volatile organic compounds (VOCs) in soil, water, and air to meet regulatory compliance, such as EPA methods. Also used in research and quality control for petrochemical and fuel testing where precise measurement of aromatic hydrocarbons is required.